Japan's Tui from wing to No.8
The Japanese face two-times World Cup champions South Africa on Saturday.
Coach Eddie Jones made five changes from the side that beat Georgia 13-10 in Gloucester on September 5, the side's last Test match.
Akihito Yamada replaces Yoshikazu Fujita on the wing and Hendrik Tui moves from the wing to No.8.
Flyhalf Harumichi Tatekawa drops to the bench in place of Kosei Ono, and Kensuke Hatakeyama replaces Hiroshi Yamashita, who is named as a replacement.
Tui's move to No.8 means there is no place for Koliniashi Holani, who is in the squad but not named in the matchday 23.
The starting 15 for Saturday has an average age of 29 years and 310 days, eclipsing the side who played in the 13-10 win over Georgia on September 5, whose average was 29 years and 299 days.
The starting side boasts 574 caps, which is the second most by a Japanese Test side after the win against Georgia, which had 586 caps.
The side is also the most experienced to play in a World Cup for Japan, beating the 465 caps held by the starting 15 for Japan's 21-47 defeat to France on 10 September 2011.
Hitoshi Ono continues to extend his record as Japan's most capped player, winning his 95th cap.
Japan: 15 Ayumu Goromaru, 14 Akihito Yamada, 13 Male Sau, 12 Craig Wing, 11 Kotaro Matsushima, 10 Kosei Ono, 9 Fumiaki Tanaka, 8 Hendrik Tui, 7 Michael Broadhurst, 6 Michael Leitch (captain), 5 Hitoshi Ono, 4 Luke Thompson, 3 Kensuke Hatakeyama, 2 Shota Horie, 1 Masataka Mikami.
Replacements: 16 Takeshi Kazu, 17 Keita Inagaki, 18 Hiroshi Yamashita, 19 Shinya Makabe, 20 Amanaki Mafi, 21 Atsushi Hiwasa, 22 Harumichi Tatekawa, 23 Karne Hesketh.
Date: Saturday, September 19
Venue: Brighton Community Stadium, Brighton
Kick-off: 16.45 (17.45 SA time, 15.45 GMT, 00.45 Sunday, September 20, Japan time)
Referee: Jérôme Garcès (France)
Assistant referees: JP Doyle (England), Federico Anselmi (Argentina)
TMO: Graham Hughes (England)
Source: @rugbyworldcup